Phloem sap proteins from Cucurbita maxima and Ricinus communis have the capacity to traffic cell to cell through plasmodesmata.
Identifieur interne : 001171 ( Main/Exploration ); précédent : 001170; suivant : 001172Phloem sap proteins from Cucurbita maxima and Ricinus communis have the capacity to traffic cell to cell through plasmodesmata.
Auteurs : S. Balachandran [États-Unis] ; Y. Xiang ; C. Schobert ; G A Thompson ; W J LucasSource :
- Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 1997.
Abstract
In angiosperms, the functional enucleate sieve tube system of the phloem appears to be maintained by the surrounding companion cells. In this study, we tested the hypothesis that polypeptides present within the phloem sap traffic cell to cell from the companion cells, where they are synthesized, into the sieve tube via plasmodesmata. Coinjection of fluorescently labeled dextrans along with size-fractionated Cucurbita maxima phloem proteins, ranging in size from 10 to 200 kDa, as well as injection of individual fluorescently labeled phloem proteins, provided unambiguous evidence that these proteins have the capacity to interact with mesophyll plasmodesmata in cucurbit cotyledons to induce an increase in size exclusion limit and traffic cell to cell. Plasmodesmal size exclusion limit increased to greater than 20 kDa, but less than 40 kDa, irrespective of the size of the injected protein, indicating that partial protein unfolding may be a requirement for transport. A threshold concentration in the 20-100 nM range was required for cell-to-cell transport indicating that phloem proteins have a high affinity for the mesophyll plasmodesmal binding site(s). Parallel experiments with glutaredoxin and cystatin, phloem sap proteins from Ricinus communis, established that these proteins can also traffic through cucurbit mesophyll plasmodesmata. These results are discussed in terms of the requirements for regulated protein trafficking between companion cells and the sieve tube system. As the threshold value for plasmodesmal transport of phloem sap proteins falls within the same range as many plant hormones, the possibility is discussed that some of these proteins may act as long-distance signaling molecules.
DOI: 10.1073/pnas.94.25.14150
PubMed: 9391168
PubMed Central: PMC28448
Affiliations:
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Balachandran</name><affiliation wicri:level="2"><nlm:affiliation>Section of Plant Biology, Division of Biological Sciences, University of California, Davis, CA 95616, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Section of Plant Biology, Division of Biological Sciences, University of California, Davis, CA 95616</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Xiang, Y" sort="Xiang, Y" uniqKey="Xiang Y" first="Y" last="Xiang">Y. Xiang</name></author><author><name sortKey="Schobert, C" sort="Schobert, C" uniqKey="Schobert C" first="C" last="Schobert">C. Schobert</name></author><author><name sortKey="Thompson, G A" sort="Thompson, G A" uniqKey="Thompson G" first="G A" last="Thompson">G A Thompson</name></author><author><name sortKey="Lucas, W J" sort="Lucas, W J" uniqKey="Lucas W" first="W J" last="Lucas">W J Lucas</name></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="ISSN">0027-8424</idno><imprint><date when="1997" type="published">1997</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In angiosperms, the functional enucleate sieve tube system of the phloem appears to be maintained by the surrounding companion cells. In this study, we tested the hypothesis that polypeptides present within the phloem sap traffic cell to cell from the companion cells, where they are synthesized, into the sieve tube via plasmodesmata. Coinjection of fluorescently labeled dextrans along with size-fractionated Cucurbita maxima phloem proteins, ranging in size from 10 to 200 kDa, as well as injection of individual fluorescently labeled phloem proteins, provided unambiguous evidence that these proteins have the capacity to interact with mesophyll plasmodesmata in cucurbit cotyledons to induce an increase in size exclusion limit and traffic cell to cell. Plasmodesmal size exclusion limit increased to greater than 20 kDa, but less than 40 kDa, irrespective of the size of the injected protein, indicating that partial protein unfolding may be a requirement for transport. A threshold concentration in the 20-100 nM range was required for cell-to-cell transport indicating that phloem proteins have a high affinity for the mesophyll plasmodesmal binding site(s). Parallel experiments with glutaredoxin and cystatin, phloem sap proteins from Ricinus communis, established that these proteins can also traffic through cucurbit mesophyll plasmodesmata. These results are discussed in terms of the requirements for regulated protein trafficking between companion cells and the sieve tube system. As the threshold value for plasmodesmal transport of phloem sap proteins falls within the same range as many plant hormones, the possibility is discussed that some of these proteins may act as long-distance signaling molecules.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">9391168</PMID><DateCompleted><Year>2008</Year><Month>04</Month><Day>18</Day></DateCompleted><DateRevised><Year>2019</Year><Month>05</Month><Day>01</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0027-8424</ISSN><JournalIssue CitedMedium="Print"><Volume>94</Volume><Issue>25</Issue><PubDate><Year>1997</Year><Month>Dec</Month><Day>09</Day></PubDate></JournalIssue><Title>Proceedings of the National Academy of Sciences of the United States of America</Title><ISOAbbreviation>Proc Natl Acad Sci U S A</ISOAbbreviation></Journal><ArticleTitle>Phloem sap proteins from Cucurbita maxima and Ricinus communis have the capacity to traffic cell to cell through plasmodesmata.</ArticleTitle><Pagination><MedlinePgn>14150-5</MedlinePgn></Pagination><Abstract><AbstractText>In angiosperms, the functional enucleate sieve tube system of the phloem appears to be maintained by the surrounding companion cells. In this study, we tested the hypothesis that polypeptides present within the phloem sap traffic cell to cell from the companion cells, where they are synthesized, into the sieve tube via plasmodesmata. Coinjection of fluorescently labeled dextrans along with size-fractionated Cucurbita maxima phloem proteins, ranging in size from 10 to 200 kDa, as well as injection of individual fluorescently labeled phloem proteins, provided unambiguous evidence that these proteins have the capacity to interact with mesophyll plasmodesmata in cucurbit cotyledons to induce an increase in size exclusion limit and traffic cell to cell. Plasmodesmal size exclusion limit increased to greater than 20 kDa, but less than 40 kDa, irrespective of the size of the injected protein, indicating that partial protein unfolding may be a requirement for transport. A threshold concentration in the 20-100 nM range was required for cell-to-cell transport indicating that phloem proteins have a high affinity for the mesophyll plasmodesmal binding site(s). Parallel experiments with glutaredoxin and cystatin, phloem sap proteins from Ricinus communis, established that these proteins can also traffic through cucurbit mesophyll plasmodesmata. These results are discussed in terms of the requirements for regulated protein trafficking between companion cells and the sieve tube system. As the threshold value for plasmodesmal transport of phloem sap proteins falls within the same range as many plant hormones, the possibility is discussed that some of these proteins may act as long-distance signaling molecules.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Balachandran</LastName><ForeName>S</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Section of Plant Biology, Division of Biological Sciences, University of California, Davis, CA 95616, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xiang</LastName><ForeName>Y</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Schobert</LastName><ForeName>C</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Thompson</LastName><ForeName>G A</ForeName><Initials>GA</Initials></Author><Author ValidYN="Y"><LastName>Lucas</LastName><ForeName>W J</ForeName><Initials>WJ</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Proc Natl Acad Sci U S A</MedlineTA><NlmUniqueID>7505876</NlmUniqueID><ISSNLinking>0027-8424</ISSNLinking></MedlineJournalInfo></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1997</Year><Month>12</Month><Day>10</Day><Hour>2</Hour><Minute>42</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1997</Year><Month>12</Month><Day>10</Day><Hour>2</Hour><Minute>43</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1997</Year><Month>12</Month><Day>10</Day><Hour>2</Hour><Minute>42</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">9391168</ArticleId><ArticleId IdType="pmc">PMC28448</ArticleId><ArticleId IdType="doi">10.1073/pnas.94.25.14150</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Plant Cell. 1992 Dec;4(12):1539-48</Citation><ArticleIdList><ArticleId IdType="pubmed">1467652</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 1995 Dec 22;270(5244):1980-3</Citation><ArticleIdList><ArticleId IdType="pubmed">8533088</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell. 1994 Mar 11;76(5):925-32</Citation><ArticleIdList><ArticleId IdType="pubmed">8124726</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 1992 Aug;4(8):915-28</Citation><ArticleIdList><ArticleId IdType="pubmed">1392601</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 1997 Feb 28;275(5304):1298-300</Citation><ArticleIdList><ArticleId IdType="pubmed">9036853</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 1993 Dec;5(12):1783-1794</Citation><ArticleIdList><ArticleId IdType="pubmed">12271056</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1992 Nov;100(3):1433-41</Citation><ArticleIdList><ArticleId IdType="pubmed">16653142</ArticleId></ArticleIdList></Reference><Reference><Citation>Planta. 1997;202(3):349-56</Citation><ArticleIdList><ArticleId IdType="pubmed">9232906</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 1993 Oct 15;268(29):21645-9</Citation><ArticleIdList><ArticleId IdType="pubmed">8408016</ArticleId></ArticleIdList></Reference><Reference><Citation>Planta. 1995;195(3):456-63</Citation><ArticleIdList><ArticleId IdType="pubmed">7766047</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 1996 Oct;32(1-2):251-73</Citation><ArticleIdList><ArticleId IdType="pubmed">8980482</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1996 Oct 29;93(22):12643-7</Citation><ArticleIdList><ArticleId IdType="pubmed">8901636</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Cell Biol. 1993 Sep;3(9):308-15</Citation><ArticleIdList><ArticleId IdType="pubmed">14731848</ArticleId></ArticleIdList></Reference><Reference><Citation>Planta. 1991 Dec;183(1):69-76</Citation><ArticleIdList><ArticleId IdType="pubmed">24193535</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 1995 Mar 10;207(2):345-53</Citation><ArticleIdList><ArticleId IdType="pubmed">7886938</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Bot. 1996 Aug;47 Spec No:1119-28</Citation><ArticleIdList><ArticleId IdType="pubmed">21245241</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li></region></list><tree><noCountry><name sortKey="Lucas, W J" sort="Lucas, W J" uniqKey="Lucas W" first="W J" last="Lucas">W J Lucas</name><name sortKey="Schobert, C" sort="Schobert, C" uniqKey="Schobert C" first="C" last="Schobert">C. Schobert</name><name sortKey="Thompson, G A" sort="Thompson, G A" uniqKey="Thompson G" first="G A" last="Thompson">G A Thompson</name><name sortKey="Xiang, Y" sort="Xiang, Y" uniqKey="Xiang Y" first="Y" last="Xiang">Y. Xiang</name></noCountry><country name="États-Unis"><region name="Californie"><name sortKey="Balachandran, S" sort="Balachandran, S" uniqKey="Balachandran S" first="S" last="Balachandran">S. Balachandran</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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